Tier 1 - Toxicity Screening

• Aim: provide hazard data for a simple risk assessment
• Quick and inexpensive toxicity screening
• 2 test methods (1 algae and 1 invertebrate) per effluent screening event
• “abbreviated” tests accepted by the EA – fewer test concentrations and replicates than standard tests

Discharges to freshwater:

Algae
Pseudokirchneriella subcapitata
72h test
endpoint: growth inhibition

cladoceran
Daphnia magna
48h hour test
endpoint: immobilisation

Discharges to marine waters:

Skeletonema costatum
72h test
endpoint: growth inhibition

Tisbe battagliai
48h hour test
endpoint: mortality

Crassostrea gigas
24h test
endpoint: larvae development

For QA/QC purposes, a Reference test is conducted concurrently with each screening test.

Teir 2