Tier 2 - Toxicity Characterisation

Aim: provide hazard data for a refined risk assessment
Invoked if Tier 1 outcome indicates an unacceptable risk of acute toxicity in the receiving environment.
Toxicity testing using same species as Tier 1
comprehensive and fully replicated test designs – statistical endpoints determined
Additional fish tests included if a defined fishery in vicinity of discharge point may be at risk

Photo: Rainbow Trout Fry

Oncorhynchus mykiss (trout)
96h test
endpoint: mortality

Photo: Scophthalmus maximus (turbot)

Scophthalmus maximus (turbot)
96h test
endpoint: mortality