Abstract

A rhabdovirus was isolated in Czechoslovakia in 1973; the character of its cytopathic action on cell cultures showed no greater differences from that of the strain described by Fijan et al in 1971. The rhabdovirus strain, isolated anew in 1974, multiplied, producing a cytopathic effect on the cellular lines FHM, BB and EPC and killing, in dependence on the dosage and method of inoculatuon, 33-.-3 to 100% of the infected carp( Cyprinus carpio )fry while giving rise to the pathognomic symptoms of the spring viraemia of carps. In this case of spring viraemia, occurring in a North-Moravian pond, the repeated virological testing and the first electron-microscopic examination proved in the tisues of diseased fish the presence of a virus with a typical rhabdovirus morphology; its average size was 155 x 90 nm.