Characterization of the first north American isolates of viral hemorrhagic septicaemia virus.
Winton, J. R., Batts, W. N., Nishizawa, T. and Stehr, C. M.
Am. Fish. Soc. Fish Health Newsl.
Following isolation of the virus from returning adult chinook salmon at Orcas Island, Wasington, by Kathleen Hopper and the recovery of virus isolates from returning adult coho salmon at Neah Bay, Wasington, by Ray Brunson, the agents had been tested with both monoclonal and polyclonal antisera against infectious hematopoietic necrosis virus (IHNV) and found negative. The chinook isolate was also tested with polyclonal antiserum against infectious pancreatic necrosis virus (IPNV) and did not react. In addition, each virus caused a cytopathic effect (CPE) in fish cell lines that was unlike that caused by other viruses isolated from Pacific salmon in North America. Because the two agents were recovered from tissue samples of chinook salmon and ovarian fluid samples of coho salmon and initially appeared different from each other in the speed and nature of their CPE, the ioslates were presumed to represent two new, or at least exotic, viruses and were referred to the National Fisheries Research Center in Seattle for assistance in determining their identity.Convincing evidence is presented for the close biochemical and antigenic relationships between these two isolates and the F 1 serotype of viral haemorrhagic septicaemia virus(VHSV ).