The isolation of a rhabdovirus, identified as pike fry rhabdovirus, from brown trout fry( Salmo trutta )is described. The fry were submitted to the laboratory for examination, many with swollen eyes and abdomens. Viral CPEs were observed in BF-2 cells and rhabdovirus particles measuring 130 x 80 nm were seen by electron microscopy. Serum neutralisation tests identified the virus as pike fry rhabdovirus. No cross-neutralisation was observed with other rhabdoviruses tested, including VHS, IHN, SVC, EVX or EVA viruses. In pathogenicity studies no mortalities were observed and no pathological lesions were seen in rainbow trout (S. gairdneri) or brown trout fry exposed to varying concentrations of the isolate (10(3.0) to 10(5.5) TCID50 - 0.05 ml).