Abstract

Detection of Haplosporidium nelsoni in Japan

(Microorganism never previously detected).

Information received by OIE on 1 October 2001 from Dr Shigeo Miyajima, Director of Animal Health Division, Ministry of Agriculture, Forestry and Fisheries, Tokyo:

Report date : 28 September 2001.

Diagnosis : please note, this information relates to the detection of the pathogen and not the disease.
A . Laboratory where detection was made: National Research Institute of Aquaculture, Fisheries Research Agency.
B . Tests used for detection: PCR(1), in situ hybridization and hematoxylin-eosin staining.
C . Result: 2% positive in hematoxylin-eosin staining (Plasmodia are recognised in 2 samples out of 100).
D . Other information: this microorganism has not been detected in adult oysters by hematoxylin-eosin staining.

Places where samples were collected: Location: North-eastern part of Japan

No. of samples : 100

Description of affected population : Pacific oyster (Crassostrea gigas) spats.

Epidemiology : no disease outbreaks associated with this microorganism have been reported.

(1) PCR: polymerase chain reaction.