The first documented appearance of infectious salmon anemia (ISA) at a US salmon farm is described. Fish presented clinically with listless behavior, exophthalmia, pale gills, and fin and skin hemorrhages. Gross necropsy findings included dark livers, swollen spleens and petechial hemorrhages of mesenteric fat and internal organs. Laboratory testing results consisted of cytopathic effect attributable to ISAV on cell culture, appropriately amplified cDNA fragments by rt-PCR, and positive-range immunochemistry results. Histopathology suggestive of ISA was observed in kidney and liver sections.