DOI: 10.14466/CefasDataHub.1

The effect of acid and neutral iodine solution on phytoplankton sample preservation


The data are the results of lab experiments conducted in 2013-2014 at the Cefas laboratory, Lowestoft, UK. Multiple marine phytoplankton taxa were preserved in one of three different Lugol's Iodine solutions (one neutral and two acidified at different strengths). Samples were enumerated over a period of 8 months to investigate degradation rates across taxa and preservatives. Samples consisted of 5 cultured species Ditylum brightwellii, Prorocentrum lima, Karenia mikimotoi, Coccolithus pelagicus & Tetraselmis suecica and one wild (mixed) sample. Each of the samples were diluted and divided equally between three plastic bottles which had been spiked with one of three Lugol's Iodine solutions (one neutral and two acidic). After 24 hrs a 5mL subsample from each bottle was settled into a 5mL Utermöhl chamber and all cells enumerated using an inverted microscope, after which the subsample was discarded. The process was repeated after 3 days, 1 week, 2 weeks, 1 month, 2 months, 4 months, 6 months and 8 months after preservation.


Williams, Oliver / Beckett, Rachel / Travell, Nicola / Walton, Alison / Crisp, Cheryl


Alga, Plankton, Phytoplankton, Laboratory, Laboratory experiment, Laboratory research, Laboratory test, Sample, Species, Taxonomy

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Williams et al. (2015). The effect of acid and neutral iodine solution on phytoplankton sample preservation. Cefas, UK. V1. doi: