Publication Abstract

Isolation and characterisation of 28 new microsatellite markers for European flounder (Platichthys flesus L.).

Niklas Tysklind, Teresa Neuparth, Gregg R. Ashcroft, Martin I. Taylor, Brett P. Lyons*, Ian D. McCarthy, Gary R. Carvalho.

European Flounder (Platichthys flesus L.) are used in ecotoxicological studies to provide detailed information on the effects of pollution on individual fish. Data on population and evolutionary level effects are, however, limited. Nuclear genetic markers are heralded to provide such information, and although there are some nuclear markers available for European flounder, higher numbers will increase the resolution of population structure patterns of this catadromous fish. Here, the isolation and characterisation of 28 species specific microsatellite loci are presented. The number of alleles ranged from 8 to 38, and observed heterozygosity from 0.542 to 1.  

Reference

Niklas Tysklind, Teresa Neuparth, Gregg R. Ashcroft, Martin I. Taylor, Brett P. Lyons*, Ian D. McCarthy, Gary R. Carvalho. (2009) Isolation and characterisation of 28 new microsatellite markers for European flounder (Platichthys flesus L.). Molecular Ecology Resources, 9 (3), 1065-1068