Publication Abstract

Evaluation of Biolog's Microlog system for the identification of Gram positive cocci pathogenic for fish

D. Verner-Jeffreys*, E. Roberts*, J. Driscoll, A. Bayley* and M. Algoet*

The ability of the commercial miniaturised phenotypic testing system Biolog GP2 Microplate™ and its supporting soware (Microlog 1) to identify a heterogeneous collection of 42 Lactococus garviae, Streptococcus iniae and other fish pathogenic Gram positive cocci (GPC) was evaluated. The strains were also identified based on sequencing of partial 16S rRNA genes. Aer 16-24h incubation, all 18 L. garvieae and 10 S. iniae isolates were correctly identified using the Biolog Microlog 1 system. The software also correctly identified the other GPC fish isolates tested, except Lactococcus piscium, that was unable to grow on the recommended proprietary agar (BUG). It is recommended that Biolog GP2 MicroplateTM, supported by confirmatory 16S rRNA gene sequencing and, where further epidemiological information is required, molecular typing, may offer a robust combinatorial GPC identification solution.

Reference:

D. Verner-Jeffreys*, E. Roberts*, J. Driscoll, A. Bayley* and M. Algoet* (2011) Evaluation of Biolog's Microlog system for the identification of Gram positive cocci pathogenic for fish. Bulletin of the European Association of Fish Pathologists 31(5) 2011, 171